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Laboratory Equipments » PCR Devices » Real Time PCR Devices » GenX-5T Real Time Fluorescence Quantitative PCR System
GenX-5T Real Time Fluorescence Quantitative PCR System

 

Features

 

> Compatible with 96x0.2mL 8-tube strips and single tubes,

> 6-zone independent temperature control to adjust different reaction conditions,

> Supports 5-color fluorescence channels and a special 6th channel.

> 7 inch high definition capacitive touch screen, easy to operate.

> Supports multiple connections to a computer and LIMS/LIS system.

 

 

Application Area


> Basic Research
In research on molecular cloning, gene expression and genotyping in scientific research institutes,


> Medical Diagnosis
Pathogen detection, genetic screening, tumor diagnosis, etc. in medical institutions.


> Food Safety
Entry-Exit and FDA inspections of foodborne pathogens, genetically modified products, meat adulteration, excessive microorganisms, etc.


> Epidemic Prevention and Control
Identification and tracking of epidemics (avian flu, SARS, dengue fever, etc.) by national systems such as CDC and Customs.

 


Technological Innovation


> Innovation in Temperature Control
Based on six-zone independent temperature control, using the high efficiency of closed liquid conduction, combines with environmental scanning monitoring and auxiliary infrared heating adjusted by intelligent frequency conversion of voltage and current system to ensure a fast ramp speed and effectively prevent Temperature overshoot without edge effect and evaporation results in better temperature uniformity and repeatability in amplification, saving time and increasing efficiency.


> Optical Detection Advantages
Representing the world's leading optical transmission and collection technology, optical fiber is used to transmit excitation and emission light to each reaction well and cooled CCD without attenuation.It improves detection sensitivity to ensure effective detection of low-copy samples while ensuring consistency and authenticity of excitation and detection.The added channel mismatch functionality extends the scope of application of qPCR to the protein level and provides a new way to generate multiple systems of diagnostic reagents.


> Improved Software
Real-time monitoring, automatic identification and calculation of positive and negative results, automatic generation of standard curve, absolute/relative quantification, multiplex quantification, melting curve, gene mutation, quality control chart analysis, fluorescence calibration, PCR amplification efficiency, etc.

 

 

 

 

Technical Specifications ;

 

 

Basic

Sample Capacity

96x0.2mL

Applicable Consumable

0.2mL Single Tube, 8x0.2mL Tube Strip, 96 Well Plate

PCR Volume Range

10-120uL

Working Temperature

15-30°C

Storage Temperature

-20-55°C

Ambient Relative Humidity

<85%

Dimensions and Weight

540x351x310mm (WxDxH), 20 Kg

Power Supply

100/240V, 50Hz, 1500VA

Temperature Control System

Heating/Cooling Method

Peltier, independent and intelligent temp. Control over 6 zones

Temperature Range

4°C-100°C

Ramp Rate

Heating : 4.2°C, Cooling : 4.2°C

Temperature Accuracy

±0.1°C

Temperature Uniformity

±0.2°C

Temperature Control Technology

6-zone independent temp. Control combined with thermal compensation tech to reduce edge effect

Gradient Temperature Function

Yes

Gradient Temperature Columns

12

Gradient Temperature Range

1°C - 32°C

Gradient Temperature Zones

6

Gradient Temperature Set Range

30°C - 100°C (Room temperature is lower than 28°C)

Detection System

Excitation Source

LED

Detection Device

PD

Light Propagation Medium

Dual-96 high temperature resistant optical fibers for space

Detection Channels

5 (Expandable to 6)

Excitation Range

1st Channel : 470nm±10nm   2nd Channel : 525nm±10nm   3rd Channel : 570nm±10nm

4th Channel : 620nm±10nm   5th Channel : 670nm±10nm   6th Channel : Customizable

Detection Range

1st Channel : 512nm-528nm   2nd Channel : 562nm-578nm   3rd Channel : 612nm-628nm

4th Channel : 662nm-678nm   5th Channel : 702nm-718nm   6th Channel : Customizable

Dye Compatibility

FAM/SYBR Green/Eva Green/LC Green/Fluorescein; VIC/HEX/TET/Cy3/JOE/Alexa555;

ROX/Cy3.5/Texas Red; Cy5/LC Red640; Cy5.5/LC Red705; Tamara

Detection Sensitivity

>1 copy

Confidence Coefficient

99.90%

Sensitivity

Allowed 1.5-fold in single reaction

Sample Detection Repeatability

CV<1%

Linear Range

1-1010

Sample Linearity

>0.99

Software

Software Language

English

Control Method

Touch screen operation, connect to PC for data analysis, multiple connections, support LIMS/LIS system

Software Function

Real-time monitoring, automatic identification and calculation of positive and negative results results, automatic establishment of standard curve, absolute/relative quantification, multiple quantification, melting curve, gene mutation, key method genotyping (Taqman probe method), Tm value determination, quality ontrol graphic analysis, PCR amplification efficiency, etc.

Output

Excel/Word/PDF

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